| Category | Small-molecule Flourescent PEG |
| Molecular Formula | C51H72N6O15S4 |
| Molecular Weight | 1137.4 |
| Shipping | Ambient temperature |
| Storage | -20°C |
Cy5 Biotin
Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Structure of Cy5 Biotin
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- Specification
| Synonyms | Cy5 Biotin |
| IUPACName | 3-[2-[(1E,3E,5E)-5-[1-[5-[2-[2-[2-[2-[5-[(3aS,4R,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethylamino]-5-oxopentyl]-3,3-dimethyl-5-sulfoindol-2-ylidene]penta-1,3-dienyl]-3,3-dimethyl-5-sulfoindol-1-ium-1-yl]propane-1-sulfonate |
| CanonicalSMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)O)[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCC(=O)NCCOCCOCCOCCNC(=O)CCCCC5C6C(CS5)NC(=O)N6)C=CC(=C4)S(=O)(=O)O)(C)C)CCCS(=O)(=O)[O-])C |
| InChI | InChI=1S/C51H72N6O15S4/c1-50(2)38-33-36(75(64,65)66)18-20-41(38)56(44(50)14-6-5-7-15-45-51(3,4)39-34-37(76(67,68)69)19-21-42(39)57(45)25-12-32-74(61,62)63)24-11-10-17-47(59)53-23-27-71-29-31-72-30-28-70-26-22-52-46(58)16-9-8-13-43-48-40(35-73-43)54-49(60)55-48/h5-7,14-15,18-21,33-34,40,43,48H,8-13,16-17,22-32,35H2,1-4H3,(H6-,52,53,54,55,58,59,60,61,62,63,64,65,66,67,68,69)/t40-,43+,48-/m0/s1 |
| InChIKey | FHRGIHYAKBLXKY-SGWHYLBPSA-N |
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