Thiol(-SH) PEG

BOC Sciences is a leading provider of PEG chemicals that are widely used in various applications such as drug delivery, bioconjugation, and surface modification. Thiol (-SH) PEGs are highly flexible compounds that have several benefits over other PEG types, including improved stability, solubility, and biocompatibility. BOC Sciences offers a range of thiol PEGs with different molecular weights, chain lengths, and functional groups to meet the varying needs of our clients. Our thiol PEGs are made using cutting-edge chemical techniques and placed through a rigorous quality control process to ensure purity and uniformity.

Thiol Functional Group

Thiol group, also known as sulfhydryl group, is a functional group formed by the combination of sulfur atoms and hydrogen atoms. Its chemical formula is -SH and it is an important component of many organic compounds. Thiol groups are found in a variety of organic compounds, including amino acids, proteins, and enzymes. One of the key chemical properties of thiol groups is their ability to undergo redox reactions. It is possible to oxidize the thiol group to create a disulfide bond, which is a covalent connection between two sulfur atoms. This process, called disulfide bond formation, is essential for the stability and function of many proteins. Reduction is another method for breaking disulfide bonds, which regenerates the thiol group and modifies the protein's structure in a reversible way.

PEG Thiol

Thiol PEG is extremely reactive and adaptable due to its special chemical characteristics. The thiol group (-SH) that is present in the structure of this substance allows it to bind to a number of different molecules, which is one of its most remarkable characteristics. Thiol PEG has the ability to crosslink molecules together by creating covalent connections between them. It can also be utilized as a surface modifier, which implies that it can change the surface characteristics of substances like proteins and nanoparticles.

Fig. 1. Application: PEG-thiol-based hydrogel with controllable properties (European Polymer Journal. 2016, 74: 1-12).

Thiol PEG is the suitable chemical for a wide range of applications because of its structural characteristics. Its structure contains a lengthy, malleable polymer chain called polyethylene glycol. PEG chains are particularly flexible due to their structural flexibility and variability, which enables them to be attached to a range of molecules and surfaces. Another key component of thiol PEG is the thiol group (-SH). Thiol-PEG is appropriate for surface modification, crosslinking, and other processes because of the high reactivity of its thiol groups, which may crosslink with a wide range of materials, including proteins and nanoparticles.

PEG Thiol Synthesis

PEG thiol reagents with different PEG chain lengths, thiol group densities, and functional groups are available from BOC Sciences for use in tissue engineering, drug delivery, bioconjugation, and diagnostics. In addition to standard PEG thiol reagents, BOC Sciences offers custom synthesis services for researchers who require specific PEG thiol reagents with unique properties. With our expertise in organic chemistry and polymer science, we can design and synthesize PEG thiol reagents with customized molecular properties, functional groups, and linker molecules.

Thiol PEG Amine

Thiol PEG Amine (HS-PEG-NH2), also known as thiol-terminated polyethylene glycol amine, is a versatile compound that has found wide application in bioconjugation and bioconjugation chemistry. The molecule features a linear chain of PEG with an amine group at one end and a thiol group at the other. Thiol PEG Amine is able to form stable thioether bonds with thiol-reactive molecules via a thiol-Michael addition reaction. This property makes it particularly suitable for the functionalization of biomolecules such as proteins and peptides, as well as surfaces, nanoparticles, and other materials. In drug delivery, thiol polyethylene glycol amines can be conjugated to therapeutic agents to increase their circulation time in the body, reduce their immunogenicity, and target specific tissues or cells. In diagnostics, thiol polyethylene glycol amines can be used to label antibodies, enzymes, or other biomolecules to detect disease biomarkers or pathogens. In biosensors, thiol polyethylene glycol amines can be attached to sensor surfaces to immobilize bioreceptors and improve their performance in sensing applications.

Thiol PEG Azide

Thiol PEG Azide (Azide-PEG-SH), also known as thiol-terminated poly(ethylene glycol) azide. Azide-PEG-SH is a popular choice for functionalizing biomolecules, nanoparticles, and surfaces due to its biocompatibility, water solubility, and ability to undergo selective chemical reactions. The thiol group of thiol PEG azide is a sulfur-containing functional group that is highly reactive toward maleimide and maleic anhydride groups. This allows thiol PEG azide to selectively conjugate to proteins, peptides, antibodies, and other biomolecules that contain maleimide or maleic anhydride groups. This thiol-maleimide or thiol-maleic anhydride reaction is often used to label biomolecules with fluorophores, biotin, or other functional groups for a variety of applications such as imaging, diagnostics, and drug delivery.

Biotin PEG Thiol

Thiol PEG Biotin (Biotin-PEG-SH) is a versatile molecule that plays a vital role in a variety of biological and chemical applications. The compound consists of a thiol group, a PEG spacer, and a biotin moiety. The biotin moiety in Biotin-PEG-SH is a small molecule that binds specifically to the protein avidin and its homologs, streptavidin, and neutravidin with high affinity. The biotin-avidin interaction is one of the strongest non-covalent interactions in nature, with a dissociation constant in the femtomolar range. This high affinity and specificity make biotin an ideal tag for labeling and purifying biomolecules and for immobilizing molecules on surfaces via biotin-avidin interactions. Biotin-PEG-SH is widely used in bioconjugation studies for the functionalization of proteins, peptides, antibodies, and other biomolecules. By attaching Biotin-PEG-SH to these biomolecules, researchers can easily introduce biotin tags for downstream applications such as protein purification, immunoassays, and imaging studies.

HS-PEG-SH

HS-PEG-SH is a compound that is a thiol-terminated polyethylene glycol with a sulfhydryl group. One of the main properties of HS-PEG-SH is its ability to form stable covalent bonds with other molecules through its thiol group. This makes it ideal for surface and biomolecule modification as well as conjugation of drugs and imaging agents. By attaching thiol-terminated PEG to a matrix or molecule, PEG chains can be effectively introduced to improve stability, solubility, and biocompatibility. In addition, its biocompatibility ensures that it can be safely used in biological systems without any adverse effects. For example, HS-PEG-SH can be used to modify the surface of scaffolds or biomaterials to improve their biocompatibility and interaction with cells. By attaching PEG chains to the surface of a scaffold, its propensity to elicit an immune response can be reduced and its integration with surrounding tissues can be improved.

What are the Uses of Thiol PEG?

Thiol PEG is superior to other chemical compounds in several ways. Thiol PEG's biocompatibility is one of its most important benefits. Thiol PEG is a substance that is safe to use in the human body and is not poisonous. This makes it the perfect substance for use in imaging, diagnostics, and drug delivery. Moreover, thiol PEG has good stability, making it appropriate for application in a range of settings. Thiol PEG can be employed in a variety of biotechnology and pharmaceutical applications since it is stable in aqueous solutions.

Thiol PEG for Drug Delivery

One of the most common applications of Thiol PEG is in drug delivery systems. Thiol PEG can be used as a binding molecule to attach drugs or other bioactive molecules to carriers, such as nanoparticles or liposomes. By attaching Thiol PEG to a carrier, the drug or molecule can be protected from degradation and cleared more slowly from the body, thereby extending circulation time and improving therapeutic efficacy. Thiol PEG can also be used to modify the surface of drug delivery carriers to improve their biocompatibility and reduce their immunogenicity, making them more suitable for in vivo applications.

Thiol PEG for Biosensors

In addition to drug delivery, Thiol PEG is also widely used in the development of biosensors. A biosensor is an analytical device that incorporates biomolecules, such as enzymes or antibodies, to detect specific analytes in a sample. Thiol PEG can be used to immobilize these biomolecules on a surface, such as a sensor chip or electrode, providing a stable and biocompatible interface for detecting the analyte. Thiol PEG-modified biosensors have been used in a wide range of applications, including medical diagnostics, environmental monitoring, and food safety testing.

Thiol PEG for Tissue Engineering

Thiol PEG is also a valuable tool in the field of tissue engineering. Tissue engineering is a rapidly growing field that seeks to create functional tissues and organs in the laboratory for transplantation or regenerative medicine applications. Thiol PEG can be used to modify the surface of scaffolds, which are porous materials that provide a structural template for tissue growth, thereby enhancing cell adhesion, proliferation, and differentiation. Thiol PEG-modified scaffolds have been used to regenerate a variety of tissues, including bone, cartilage, and skin, with promising results.

Thiol PEG for Surface Modification

Another important application of thiol PEG is surface modification. Thiol PEG can be used to functionalize surfaces with a variety of functions, including antibodies, enzymes, and fluorescent dyes, which can be used in applications such as protein immobilization, cell patterning, and biosensing. Thiol PEG-modified surfaces are biocompatible and resistant to protein fouling, making them ideal for biotechnology and biomedical research applications.

Reference

1. Havazelet, B. et al. PEG-Thiol based hydrogels with controllable properties. European Polymer Journal. 2016, 74: 1-12.