PEGylation of Lipids
PEG lipids, also known as pegylated lipids, are a class of PEG derivatives attached to lipid moieties such as DMG or DSPE. Pegylation is a well-established strategy for increasing the target specificity, circulation time, and stability of lipids, thereby enhancing their use in drug development and drug delivery. BOC Sciences is committed to providing comprehensive PEGylated lipid support services to facilitate your drug delivery, gene delivery and tissue engineering programs.
What are PEG-Lipid Conjugates?
Lipid molecules consist of a hydrophilic head and a hydrophobic tail, and in the presence of an aqueous medium, lipids self-assemble to form liposomes. The presence of a hydrophilic aqueous core and hydrophobic lipid bilayer regions enables them to encapsulate both hydrophobic and hydrophilic molecules, making them ideal candidates for vaccine and drug delivery as compared to metallopolymer and dendrimer-based carriers. ideal candidate. PEG (Polyethylene glycol) is more popular due to its non-ionic, biocompatibility, hydrophilicity, and easy synthesis. PEGylated lipids consist of PEG chains, linkers, and hydrophobic anchors. One of the ends of the PEG chains in PEGylated lipids is attached to the hydrophobic anchor by a linker.
Brief Strategies for Lipid PEGylation
PEGylation of lipids involves the incorporation of PEG-conjugated phospholipids into the liposome bilayer or the grafting of PEG using functionalized lipids containing primary amino, epoxy, or diacylglycerol moieties. PEG-conjugated phospholipids can be synthesized by using PEG derivatives reactive towards the primary amines of phosphatidylethanolamine (PE). PEGylated liposomes are simply prepared by mixing these PEG-conjugated lipids in the lipid mixture during liposome preparation or incubation with preformed liposomes. In an alternative approach, lipophilic compounds bearing reactive groups such as amino and carboxyl groups can also be used to create PEGylation sites on the liposome surface. By incorporating these components into the bilayer, 500-2,000 reactive functional groups can be introduced for reaction with various PEGylation reagents.
Fig. 1. Regimes in a PEG coated liposome a) Interdigitated Mushroom; b) Mushroom Regime; c) Brush Regime (International Journal of Lipids. 2020, 1(2): 1-20).
PEG is often covalently bound to lipid molecules and mixed with other lipids to form lipid-based nanoparticles. The chain mobility of PEG molecules can provide hydration during circulation and avoid non-specific adsorption of components in tissues and body fluids. This approach helps the nanoparticles prolong their circulation and avoid absorption by the reticuloendothelial system, allowing them to travel safely through the body. Furthermore, the density of PEG on the surface of lipid-based nanoparticles is critical for the design of these nanocarriers. As the density of PEG-grafted on the liposome surface increased, the half-life also increased, which was attributed to non-specific uptake of proteins and decreased uptake by the reticuloendothelial system. The low incorporation of PEG does not fully protect the nanoparticles from interacting with serum proteins because PEG does not provide the desired steric stability to the nanoparticles. Therefore, for mechanical stability reasons, the proportion of PEG in lipid bilayers is usually lower than 5-6%.
Our Lipid PEGylation Capabilities
BOC Sciences can provide a variety of PEG-modified liposome derivatives with different molecular weights and functional groups. The molecular weight of our commonly used PEG is from 600-12000, including the most commonly used PEG from 2000 to 5000. Our PEGylated lipid capabilities include:
PEGylation of Liposomes | PEGylation of Sterols |
PEGylation of Phospholipids | PEGylation of Sphingolipids |
PEGylation of DSPE | PEGylation of Fluorescent Lipids |
PEGylation of Cholesterol | PEGylation of Glycolipidsl |
PEGylation of DMG | PEGylation of Photoswitchable Lipids |
References
- Misra, A. et al. PEG - A versatile conjugating ligand for drugs and drug delivery systems. Journal of Controlled Release. 2014, 192: 67-81.
- Kalyanram, P. et al. Understanding the Stealth Properties of PEGylated lipids: A Mini-Review. International Journal of Lipids. 2020, 1(2): 1-20.
Why BOC Sciences?
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Large Stock
More than 2000+ products in inventory
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Global Delivery
Warehouses in multiple cities to ensure fast delivery
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mg to kg
Multi specification for academic research and industrial production
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24/7 Technical Support
Strict process parameter control to ensure product quality
Technical Support
- Aqueous Two-Phase System (ATPS) Technique
- Capillary Electrophoresis (CE) Technique
- Enzyme-linked immunosorbent assay (ELISA) Technique
- High performance liquid chromatography (HPLC) Technique
- Hydrophobic Interaction Chromatography (HIC) Technique
- PEGylated Protein Purification Techniques
- Radiolabeling Technique
- SDS-PAGE Technique
- Ultrafiltration Technique
Products
- Lipids
- PEG Derivatives by Structure
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PEG Derivatives by Functional Group
- Acrylate/Acrylamide/Methacrylate PEG
- Aldehyde (Ald/CHO)PEG
- Alkyne PEG
- Amino PEG, PEG amine(-NH2)
- Azide PEG, Azido PEG(-N3)
- Biotin PEG
- Boc/Fmoc protected amine PEG
- Carboxylic Acid(-COOH) PEG
- Cholesterol PEG
- DBCO PEG
- DSPE PEG
- Epoxide glycidyl ether PEG
- FITC PEG
- Halide (chloride, bromide) PEG
- Hydrazide PEG
- Hydroxyl(-OH) PEG
- Maleimide(-MAL) PEG
- Multi-Arm PEG
- NHS ester PEG
- Nitrophenyl carbonate (NPC) PEG
- Norbornene PEG
- Olefin/Alkene/Vinyl PEG
- Orthopyridyl disulfide (OPSS) PEG
- Phosphate PEG
- Rhodamine PEG
- SCM PEG
- Silane PEG
- Sulfonate (tosyl, mesyl, tresyl) PEG
- tert-Butyl protected carboxylate PEG
- Thiol(-SH) PEG
- Vinylsulfone PEG
- PEG Copolymers
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PEG Raw Materials
- Small-molecule Polyethylene Glycol
- Polyethylene Glycol 1000
- Polyethylene Glycol 10000
- Polyethylene Glycol 1500
- Polyethylene Glycol 200
- Polyethylene Glycol 2000
- Polyethylene Glycol 20000
- Polyethylene Glycol 400
- Polyethylene Glycol 4000
- Polyethylene Glycol 600
- Polyethylene Glycol 6000
- Polyethylene Glycol 800
- Polyethylene Glycol 8000
Services
- PEGylation Services
- PEGylation Analysis and Method Verification
- Surface Modification and Functionalization
- PEGylated Biodegradable Polymers Synthesis
- Monodisperse PEG Synthesis
- Custom Synthesis PEG Derivatives
- PEG cGMP Manufacture
- PEG Crosslinking Services
Resources
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Technical Information
- Aqueous Two-Phase System (ATPS) Technique
- Capillary Electrophoresis (CE) Technique
- Enzyme-linked immunosorbent assay (ELISA) Technique
- High performance liquid chromatography (HPLC) Technique
- Hydrophobic Interaction Chromatography (HIC) Technique
- Introduction of Polyethylene Glycol (PEG)
- Ion Exchange Chromatography (IEX) Technique
- PEG for Chemical Synthesis
- PEG for Cosmetic Application
- PEG for Drug Delivery
- PEG for Imaging Diagnosis
- PEG for Pharmaceutical Preparation
- PEG for Tissue Engineering
- PEGylated Protein Purification Techniques
- Radiolabeling Technique
- SDS-PAGE Technique
- Size Exclusion Chromatography (SEC) Technique
- Ultrafiltration Technique
- Industry News
Our Feature
BOC Sciences supplies a unique variety of PEG derivatives and functional PEG polymers. Our products offer the most diverse collection of reactivity, ready-to-use functionality, and molecular weight options that you will not find anywhere else.

PEGylation of Peptides
and Proteins
Reduce the Immunogenicity of Peptide/Protein Drugs
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APPLICATIONS

PEG linkers For Drug
Improved Circulation Half-Life
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