PEGylation Analysis and Method Verification

BOC Sciences provides comprehensive PEGylation analysis services in terms of qualitative assessment, quantitative assessment and in vivo & in vitro characterization.

After PEGylation, conjugates will show changes in physiochemical properties, such as surface charge, hydrodynamic diameter, hydrophilicity and protein binding capability. By monitoring changes in these physiochemical properties, PEGylation can be confirmed.

Qualitative Assessments

Quantitative Assessments

In addition to using the analytical measurement mentioned above alone, we are also able to utilize the combined techniques, such as combination of ELSD and LC-MS, NIR spectroscopy coupled with principal component analysis (PCA) for characterization of hydrophilic/hydrophobic balance of PEGylated nanoparticles.

In Vitro and In Vivo Characterizations

• In vitro methods include measurement of protein adsorption, measurement of complement consumption, macrophage uptake studies, and the analysis of release rate or content stability.
• The most general in vivo assessments include pharmacokinetic profiling (Radiolabeling, Fluorescence labeling, Bioactivity Assay), tissue distribution studies and vascular permeability studies.

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Reference

1. Santos, J. H. P. M.; Torres-Obreque, K. M.; et al. Protein PEGylation for the design of biobetters: from reaction to purification processes. Brazilian Journal of Pharmaceutical Sciences 2018, 54 (SPE).