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PEGylation Analysis and Method Verification
BOC Sciences provides comprehensive PEGylation analysis services in terms of qualitative assessment, quantitative assessment and in vivo & in vitro characterization.
After PEGylation, conjugates will show changes in physiochemical properties, such as surface charge, hydrodynamic diameter, hydrophilicity and protein binding capability. By monitoring changes in these physiochemical properties, PEGylation can be confirmed.
Qualitative Assessments
| Method | Analysis Content |
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Purity of PEGylated proteins and the extent of PEGylation |
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Hydrodynamic diameter, zeta potential and size distribution of PEGylated species |
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The size of PEGylated proteins |
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Secondary and tertiary structural conformation of native and PEGylated proteins. |
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Qualitative assessment of PEGylation relies on the presence of CH2 and C-O-C absorption bands. |
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The surface charge of PEGylated species |
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Qualitative assessment of PEGylation relies on the presence or absence of specific chemical moieties |
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Qualitative assessment of the structural conformations of the lipid PEGylated nanocarriers |
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The morphology of PEGylated nanocarriers |
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Identification the density and spatial distribution of PEG polymers on the surface of nanocarriers |
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Identification the density and spatial distribution of PEG polymers on the surface of nanocarriers |
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Evaluation of the degree of PEG substitution |
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Quantitative Assessments
| Method | Analysis Content |
|---|---|
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The amount of PEG associated with colloidal carriers |
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The concentration of PEGylated proteins in serum samples |
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The concentration and localization of PEG chains |
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The amount of attached PEG polymer |
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The molecular mass of PEGylated species |
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The molecular mass of PEGylated species |
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The average molecular weight of PEGylated species and degree of PEGylation |
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The average molecular weight of PEGylated species and degree of PEGylation |
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Changes of elemental composition on the conjugate surface before and after PEGylation |
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Determination of PEG content and conformation |
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The content of PEG |
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The quantitative measurement of metal-containing PEGylated pharmaceuticals or nanoparticles |
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In addition to using the analytical measurement mentioned above alone, we are also able to utilize the combined techniques, such as combination of ELSD and LC-MS, NIR spectroscopy coupled with principal component analysis (PCA) for characterization of hydrophilic/hydrophobic balance of PEGylated nanoparticles.
In Vitro and In Vivo Characterizations
- In vitro methods include measurement of protein adsorption, measurement of complement consumption, macrophage uptake studies, and the analysis of release rate or content stability.
- The most general in vivo assessments include pharmacokinetic profiling (Radiolabeling, Fluorescence labeling, Bioactivity Assay), tissue distribution studies and vascular permeability studies.
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How to Order?
Reference
- Santos, J. H. P. M.; Torres-Obreque, K. M.; et al. Protein PEGylation for the design of biobetters: from reaction to purification processes. Brazilian Journal of Pharmaceutical Sciences 2018, 54 (SPE).
Why BOC Sciences？
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Large Stock
More than 2000+ products in inventory
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Global Delivery
Warehouses in multiple cities to ensure fast delivery
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mg to kg
Multi specification for academic research and industrial production
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24/7 Technical Support
Strict process parameter control to ensure product quality
Technical Support
- Aqueous Two-Phase System (ATPS) Technique
- Capillary Electrophoresis (CE) Technique
- Enzyme-linked immunosorbent assay (ELISA) Technique
- High performance liquid chromatography (HPLC) Technique
- Hydrophobic Interaction Chromatography (HIC) Technique
- PEGylated Protein Purification Techniques
- Radiolabeling Technique
- SDS-PAGE Technique
- Ultrafiltration Technique
Products
- PEG Derivatives by Structure
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PEG Derivatives by Functional Group
- Acrylate/Acrylamide/Methacrylate PEG
- Aldehyde (Ald/CHO)PEG
- Alkyne PEG
- Amino PEG, PEG amine(-NH2)
- Azide PEG, Azido PEG(-N3)
- Biotin PEG
- Boc/Fmoc protected amine PEG
- Carboxylic Acid(-COOH) PEG
- Cholesterol PEG
- DBCO PEG
- DSPE PEG
- Epoxide glycidyl ether PEG
- FITC PEG
- Halide (chloride, bromide) PEG
- Hydrazide PEG
- Hydroxyl(-OH) PEG
- Maleimide(-MAL) PEG
- NHS ester PEG
- Nitrophenyl carbonate (NPC) PEG
- Norbornene PEG
- Olefin/Alkene/Vinyl PEG
- Orthopyridyl disulfide (OPSS) PEG
- Phosphate PEG
- Rhodamine PEG
- SCM PEG
- Silane PEG
- Sulfonate (tosyl, mesyl, tresyl) PEG
- tert-Butyl protected carboxylate PEG
- Thiol(-SH) PEG
- Vinylsulfone PEG
- PEG Copolymers
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PEG Raw Materials
- Small-molecule Polyethylene Glycol
- Polyethylene Glycol 1000
- Polyethylene Glycol 10000
- Polyethylene Glycol 1500
- Polyethylene Glycol 200
- Polyethylene Glycol 2000
- Polyethylene Glycol 20000
- Polyethylene Glycol 400
- Polyethylene Glycol 4000
- Polyethylene Glycol 600
- Polyethylene Glycol 6000
- Polyethylene Glycol 800
- Polyethylene Glycol 8000
Services
- PEGylation Services
- PEGylation Analysis and Method Verification
- PEGylated Biodegradable Polymers Synthesis
- Surface Modification and Functionalization
- Monodisperse PEG Synthesis
- Custom Synthesis PEG Derivatives
- PEG GMP Manufacture
Resources
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Technical Information
- Aqueous Two-Phase System (ATPS) Technique
- Capillary Electrophoresis (CE) Technique
- Enzyme-linked immunosorbent assay (ELISA) Technique
- High performance liquid chromatography (HPLC) Technique
- Hydrophobic Interaction Chromatography (HIC) Technique
- Introduction of Polyethylene Glycol (PEG)
- Ion Exchange Chromatography (IEX) Technique
- PEG for Chemical Synthesis
- PEG for Cosmetic Application
- PEG for Drug Delivery
- PEG for Imaging Diagnosis
- PEG for Pharmaceutical Preparation
- PEG for Tissue Engineering
- PEGylated Protein Purification Techniques
- Radiolabeling Technique
- SDS-PAGE Technique
- Size Exclusion Chromatography (SEC) Technique
- Ultrafiltration Technique
- Industry News
Our Feature
BOC Sciences supplies a unique variety of PEG derivatives and functional PEG polymers. Our products offer the most diverse collection of reactivity, ready-to-use functionality, and molecular weight options that you will not find anywhere else.
