Biotin PEG

Biotin PEG is a biotin-conjugated polyethylene glycol (PEG) molecule that is widely used in life science research and drug development. PEG is a water-soluble polymer that can be covalently attached to various molecules, including drugs, proteins and peptides, to improve their solubility, stability, pharmacokinetics, and immunogenicity. Biotin is a small molecule that binds specifically to avidin or streptavidin, two proteins commonly used in biotechnology applications. BOC Sciences, a pioneering entity in the chemical synthesis, has ingeniously devised a highly potent and versatile biotin-PEG conjugation platform that provides immense value to researchers and producers alike.

Chemical Properties of Biotin PEG

The complex molecule known as biotin PEG is made up of numerous parts, each of which has unique chemical characteristics. The ethylene oxide units that make up the PEG portion of the molecule can range in length from a few to hundreds. The longer the PEG chain, the more hydrophilic and flexible the molecule becomes, which can enhance its biocompatibility and reduce its immunogenicity. The biotin part of the molecule is a cyclic ring structure that contains a sulfur atom, which can form a strong bond with the nitrogen atom of the lysine residue in proteins. The biotin-lysine bond is stable and specific, and can be used to label, purify, or immobilize proteins in various assays and applications.

Universal biotin-PEG-linked gold nanoparticle probes for the simultaneous detection of nucleic acids and proteinsFig. 1. Universal biotin-PEG-linked gold nanoparticle probes for the simultaneous detection of nucleic acids and proteins (Bioconjug. Chem. 2016: 203-211).

Structural Characteristics of Biotin PEG

Biotin PEG has several structural characteristics that make it a unique and versatile molecule. First, biotin PEG can be synthesized with different PEG lengths, biotin positions, and linker chemistries, which can affect its solubility, stability, and reactivity. For example, biotin PEG can be synthesized with a maleimide linker, which can react specifically and covalently with cysteine residues in proteins, or with a NHS ester linker, which can react with primary amines in proteins. Second, biotin PEG can be conjugated to different molecules, such as proteins, peptides, drugs, and nanoparticles, which can expand its applications and functions. Third, biotin PEG can be used in various formats, such as biotinylated beads, plates, membranes, or microarrays, which can enable high-throughput and multiplexed assays.

Application of Biotin PEG

Due to its distinctive characteristics and functionalities, biotin PEG has a wide range of applications in life science research and medication development. Protein labeling, which includes binding biotin PEG to a protein of interest, either directly or indirectly, and then detecting or purifying the labeled protein using streptavidin or avidin, is one of the most popular uses of biotin PEG. Protein-protein interactions, protein localisation, and protein expression can all be studied using this technique, which is popular in biochemistry, cell biology, and proteomics. Drug conjugation, which includes adding biotin PEG to a therapeutic molecule to enhance its solubility, stability, and targeting, is another use for biotin PEG. The effectiveness of cancer therapy, immunotherapy, and gene therapy can all be improved with this technique.

Advantages of Biotin PEG

Biotin PEG is an unparalleled labeling and conjugation method that is preferred by numerous researchers and manufacturers owing to its manifold advantages. The multifaceted nature of biotin PEG is attributed to its exceptional specificity and sensitivity, which stem from the robust and enduring biotin-lysine bond, rendering it impervious to the effects of varying conditions such as pH, temperature, and denaturants. Additionally, biotin PEG stands out among other methods owing to its intrinsic versatility and customizability, which is facilitated by the diverse range of PEG lengths, biotin positions, and linker chemistries that enable the optimization of its properties and functions for diverse applications. Notably, the biotin PEG method is highly compatible with an array of detection and purification methods such as fluorescence, chemiluminescence, and magnetic separation, thus facilitating high sensitivity, low background, and facile handling. Furthermore, the biocompatibility and non-immunogenicity of biotin PEG method, underpinned by the hydrophilic and non-toxic nature of PEG, significantly reduces the risk of adverse effects and enhances the efficacy and safety of the labeled or conjugated molecules.

Biotin PEG

Due to its distinct qualities and functionalities, biotin PEG is a potent and useful tool for life science research and medication development. A wide range of biotin PEG products, including biotin PEG linkers, multi-armed biotin-PEG, small-molecule biotin PEG, and functionalized biotin PEG, have been created by BOC Sciences to fulfill the various needs and specifications of researchers and producers. Whether you need to label, purify, or conjugate proteins, peptides, or drugs, biotin PEG can provide a reliable and efficient solution that can enhance your research and development.


  1. Scott, A.W. et al. Universal Biotin-PEG-Linked Gold Nanoparticle Probes for the Simultaneous Detection of Nucleic Acids and Proteins. Bioconjug. Chem. 2016: 203-211.

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