Standards

What are Lipidomic Standards?

A lipidomic standard is a compound or mixture of compounds with known concentration, composition and purity. It is a reference material that can be used for quantitative analysis, quality control and method validation to ensure the accuracy and reliability of analytical results. Lipidomic standard are generally assayed with high-precision instruments and the concentration is determined in the form of a standard curve or internal standard method.

Fig. 1. List of lipid standards (Analytical and Bioanalytical Chemistry. 2020, 412: 4313-4325).

Lipidomics is the study of the lipid composition of living organisms, which can play an important role in the identification of lipid biomarkers of diseases, disease diagnosis, and drug target research. Mass spectrometry is the core research tool in the field of lipidomics, while standards are one of the important basic materials for analysis and testing. BOC Sciences provides lipidomics standards for drug discovery, food testing, microbiology testing, clinical diagnostic testing, and prognostic biomarker discovery.

Types of Lipidomic Standards

According to the different preparation methods and uses of lipid standards, they can be divided into the following categories:

Single lipid standards. It mainly includes the standard substances of single components such as lipoic acid, triglyceride, phospholipid, cholesterol and so on.

Mixed lipid standards. Mixed by a variety of lipid components in accordance with a certain ratio, can be used for the analysis of the content of lipid components in complex samples.

Isotope lipid standards. Isotope labeling of certain atoms or groups in a lipid molecule to facilitate the tracking of lipid metabolism, transport and synthesis, which can be used in the study of metabolic kinetics and lipid synthesis pathways.

Lipidomics Analysis

When performing lipid analysis, it is necessary to select the appropriate analytical platform for lipidomics analysis, and commonly used methods include mass spectrometry. Then, using the corresponding standards, the lipids are analyzed qualitatively and quantitatively. During the process, professional lipidomics analysis software can be used for processing.

Lipidomics Applications

The analysis of lipidomics presents an intricate and multifaceted approach, offering a myriad of possibilities for the discovery of biomarkers in research and the exploration of pharmacological interventions across diverse disease spectra. This intricate process delves into the convoluted web of lipid metabolism, shedding light on the intricate interplay between drugs and lipid pathways, ultimately unearthing potential therapeutic targets for an array of ailments. Moreover, the expansive realm of lipidomic analysis extends its reach into the domain of nutritional research, adding further layers of complexity and depth to its already intricate tapestry.

Quality Control

The utilization of lipidomic standards in experimental settings serves as a pivotal mechanism for ensuring the stringent quality control necessary for scientific inquiry. Through meticulous comparison with unknown samples, these standards play a crucial role in safeguarding the precision and dependability of the analyzed outcomes, thereby catalyzing a marked enhancement in the reproducibility and comparability of experimental endeavors.

Standard Curves

The employment of lipidomic standards in experimental settings is instrumental in the establishment of standard curves, a pivotal tool for conducting quantitative analyses and ascertaining the concentrations of enigmatic samples. This process not only aids in delineating the concentration range of unknown samples but also serves as a catalyst for refining the precision and accuracy of experimental findings, thereby bolstering the reliability and robustness of scientific inquiry.

Instrument Calibration

The ubiquitous applicability of lipidomic standards across diverse instruments underscores their pivotal role as universal benchmarks for calibration, thereby facilitating the seamless comparison and validation of results obtained from disparate laboratories and instruments. This harmonization ensures the robustness and veracity of findings, contributing to the global standardization and validation of lipidomic analyses.

The indispensable role of lipidomic standards in guaranteeing the precision and dependability of analytical outcomes, enhancing the accuracy and consistency of analytical methodologies, and catalyzing fundamental and practical research cannot be overstated. BOC Sciences can provide customers with high-quality lipidomic standards and follow-up technical support. If you are interested in our products, please feel free to contact us.

Reference

1. Xu, M. et al. Evaluation of LC-MS and LC&LC-MS in analysis of zebrafish embryo samples for comprehensive lipid profiling. Analytical and Bioanalytical Chemistry. 2020, 412: 4313-4325.