Toolbox

Lipidomic toolbox refers to the standard products for lipidomic analysis, the main types of which include derivatives of cholesterol, bile acids, etc., which can be used to analyze lipid metabolic pathways, assess the rate of metabolism and excretion pathways, etc. BOC Sciences, as a supplier of lipidomic toolbox, offers a wide range of lipidomic toolbox products to our customers. These products can be used for lipidomics applications such as biomedical research, nutritional research, drug discovery, and more.

Fig. 1. Mass spectrometry-based lipidomics analysis strategy (Accounts Chem. Res. 2016, 49(9): 1596-1604).

Lipidomics Definition

Lipidomics is an emerging discipline for the systematic analysis of lipids in organisms and is an important branch of metabolomics. Based on liquid chromatography mass spectrometry (LC-MS), all lipid molecules within a biological sample, such as cells, tissues, organs, or body fluids, can be detected without bias and as many as possible. And then, the experimental and control groups are compared and analyzed, and differential lipid molecules are screened by statistical analysis. In this process, the relative relationship between changes in lipid metabolism and physiological and pathological changes is searched for, and ultimately the mechanism of the role of lipids in various life activities can be revealed.

Examples of Lipidomic Toolbox

Glycolithocholic acid-[2,2,4,4-d4]

Glycolithocholic acid-[2,2,4,4-d4] is the labelled analogue of Glycolithocholic acid. Glycolithocholic acid is a bile acid-glycine conjugate that can inhibits sialyltransferase. Glycolithocholic acid-[2,2,4,4-d4] can be used as an internal standard or tracer in metabolic studies. By administering an animal or cellular labeling compound, the metabolic pathways and metabolites can be traced in vivo.

Tauroursodeoxycholic Acid-[d4] Sodium

Tauroursodeoxycholic Acid-[d4] Sodium is a labeled salt of tauroursodeoxycholic acid. Tauroursodeoxycholic Acid-[d4] Sodium is used in lipidological studies, particularly related to bile acid metabolism, lipid metabolism, and the study of lipid-related diseases.

3β,5α,6β-Trihydroxycholestane-[d7]

3β,5α,6β-Trihydroxycholestane-[d7] is a labeled analog of 3β,5α,6β-trihydroxycholestane, a cholesterol impurity. 3β,5α,6β-Trihydroxycholestane-[d7] is labeled with the site of [d7], which allows for accurate determination of metabolites by mass spectrometry in terms of their relative abundance and metabolic pathways. The use of 3β,5α,6β-Trihydroxycholestane-[d7] as a tracer allows us to follow the process of cholesterol synthesis, metabolism, and transport, and to gain a deeper understanding of the regulatory mechanisms of cholesterol metabolic pathways.

Significance of Lipidomics Analysis

• Lipidomics analysis can help to reveal the lipid composition of different tissues, cells or physiological states in an organism.
• Lipidomics analysis can provide information about lipid metabolic pathways and metabolites. By parsing lipidomics data, it is possible to understand the activity of different metabolic pathways, the accumulation of metabolites and metabolic abnormalities.
• Lipidomics analysis can identify lipid markers associated with specific physiological or pathological states by comparing samples from different groups or disease states.

BOC Sciences not only provides various types of lipids, such as fluorescent lipids, natural lipids, and lipid formulations, but is also able to provide custom synthesis services for lipids that are not on the list to meet the specific needs of our customers. Our goal is to provide our customers with high-quality lipid products and exclusive product solutions. If you have any needs for lipids not listed and would like more detailed information about our products, please feel free to contact us.

Reference

1. Ryan, E. et al. Chemical derivatization and ultrahigh resolution and accurate mass spectrometry strategies for "shotgun" lipidome analysis. Accounts Chem. Res. 2016, 49(9): 1596-1604.