PEGylation of Enzymes

As a premier provider of PEGylation services for enzymes, BOC Sciences is dedicated to utilizing its cutting-edge research and state-of-the-art technology to deliver top-tier solutions to our clients. PEGylation, a cutting-edge technique that involves the modification of enzymes with PEG, has proven to be a game-changer in the biotech industry, since it enables a wide array of enzymes to exhibit improved pharmacokinetics, enhanced solubility, and superior stability. Our innovative PEGylation technology has been hailed as a remarkable advancement in the field of enzyme modification because of it has allowed for a much more efficient and effective delivery of therapeutic enzymes to the human body.

What is Enzymatic PEGylation?

Enzymatic PEGylation is a commonly used modification technique in the pharmaceutical industry and medical diagnostics. Typically, enzymes are pegylated by attaching a PEG molecule to an enzyme molecule by forming a covalent bond. This process holds tremendous promise for enhancing the pharmacokinetic properties of proteins and enzymes, such as their solubility, immunogenicity, stability, and half-life in the bloodstream. Notably, the PEGylation process can exert a profound impact on the activity, specificity, and stability of the enzyme. Depending on the degree of modification and the site of PEG attachment, the properties of the enzyme can vary widely. In some cases, PEGylation can lead to a reduction in the enzyme's catalytic activity, yet this inhibition can be alleviated by selecting optimal attachment sites on the enzyme surface.

Fig. 1. Research applications of enzymatic pegylation (Bioconjugate Chem. 2022, 33(1): 97-104).

Enzymatic PEGylation Methods

PEGylation of enzyme molecules can be achieved through either chemical or enzymatic methods. In the chemical approach, PEG molecules are affixed to enzymes via reactive chemical groups, which include carboxyl, amine, and thiol groups. Conversely, in the enzymatic method, PEG molecules are connected to enzymes through specific enzymes that selectively target certain amino acid residues located on the surface of the enzyme. The resulting PEGylated enzymes offer a plethora of applications within various fields such as medicine, biotechnology, and industry. Notably, they can be used as therapeutics for enzyme replacement therapy, cancer treatment, and metabolic disorders. Additionally, PEGylated enzymes have a pivotal role in industrial processes, for instance, food processing, detergents, and biocatalysis.

Our Enzyme PEGylation Services

At BOC Sciences, we pride ourselves on offering an extensive selection of PEGylation services for enzymes that boast a high level of perplexity. Our team of experts specializes in site-specific conjugation, controlled PEGylation, and PEGylation optimization, with a vast array of experience in this field that allows us to help you optimize your enzyme for specific applications - whether that's for therapeutic or diagnostic use or any other scientific pursuit.

What sets our PEGylation services apart is the use of state-of-the-art technologies and high-quality PEG reagents to ensure the highest level of quality and consistency, making sure your enzyme is at peak performance at all times. Our PEGylation process is fully customizable, allowing us to tailor our services to meet the specific needs of each client, making the experience as personal as possible.

In addition to our outstanding PEGylation services, we offer an impressive range of enzyme-related services that add another layer of burstiness to our offerings. Our team is equipped to provide top-notch enzyme purification, characterization, and assay development services, helping us to provide our clients with comprehensive solutions for all their enzyme-related needs, from discovery to commercialization.

Advantages of PEGylated Enzymes

• Increased stability: PEGylation improves the stability of enzymes by protecting them from proteolytic degradation and denaturation, leading to increased half-life and better shelf life.
• Reduced immunogenicity: PEGylation reduces the immunogenicity of enzymes by masking the epitopes that trigger an immune response, leading to reduced risk of adverse reactions.
• Improved solubility: PEGylation improves the solubility of enzymes by increasing their hydrophilicity, leading to better bioavailability and efficacy.
• Enhanced pharmacokinetics: By decreasing the clearance rate and lengthening the circulation duration of enzymes, PEGylation enhances their pharmacokinetics, resulting in a prolonged release and better therapeutic efficacy.
• Reduced toxicity: PEGylation improves the tolerability and safety of enzymes by reducing their contact with the host immune system.
• Improved targeting: PEGylation can also be used to direct enzymes to particular organs or cells, improving their effectiveness and minimizing their negative consequences.

We at BOC Sciences are dedicated to providing our customers with high-quality services and products that satisfy their needs. To find out more about our PEGylation services and how we can assist you with optimizing your enzyme for your particular application, get in touch with us right away.

Reference

1. Lorenz, M. et al. Chemo-Enzymatic PEGylation/POxylation of Murine Interleukin-4. Bioconjugate Chem. 2022, 33(1): 97-104.