Azido-PEG5-alcohol

Azido-PEG5-alcohol is a PEG-containing bioorthogonal conjugation building block that provides an azide handle for modular click-chemistry workflows. The molecule can be used to introduce a click-reactive group onto an antibody, peptide, linker intermediate, or payload-containing component, depending on the chemistry of its second terminal functionality. Its additional reactive group is azide bioorthogonal handle; requires complementary click handle on the antibody. The structure includes peg5 spacer to provide hydrophilic spacing. Conjugation can be performed using spaac with dbco/bcn-functionalized partner or cuaac with terminal alkyne partner, depending the complementary handle. The sequence and orientation of the coupling steps should be selected according to the functional groups carried by the two conjugation partners.