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Acid-PEG13-NHS ester

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Category Acid-PEGn-NHS ester
Catalog NO. BPG-0054
Product Name Acid-PEG13-NHS ester
CAS 2152679-62-2
Molecular Formula C34H61NO19
Molecular Weight 787.85
Acid-PEG13-NHS ester
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Description Acid-PEG13-NHS ester is a PEG linker containing a carboxylic acid and an NHS ester. The hydrophilic PEG spacer increases solubility in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
Synonyms Acid-C2-PEG13-NHS ester; COOH-PEG13-SPA; COOH-PEG11-NHS; 4,7,10,13,16,19,22,25,28,31,34,37,40-Tridecaoxatritetracontanedioic acid, 1-(2,5-dioxo-1-pyrrolidinyl) ester; 4,7,10,13,16,19,22,25,28,31,34,37,40-Tridecaoxatritetracontan-1-oic acid, 43-[(2,5-dioxo-1-pyrrolidinyl)oxy]-43-oxo-; 43-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-43-oxo-4,7,10,13,16,19,22,25,28,31,34,37,40-tridecaoxatritetracontan-1-oic acid; 1-(2,5-Dioxo-1-pyrrolidinyl) 4,7,10,13,16,19,22,25,28,31,34,37,40-tridecaoxatritetracontanedioate
IUPAC Name 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[3-(2,5-dioxopyrrolidin-1-yl)oxy-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoic acid
Canonical SMILES C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(=O)O
InChI InChI=1S/C34H61NO19/c36-31-1-2-32(37)35(31)54-34(40)4-6-42-8-10-44-12-14-46-16-18-48-20-22-50-24-26-52-28-30-53-29-27-51-25-23-49-21-19-47-17-15-45-13-11-43-9-7-41-5-3-33(38)39/h1-30H2,(H,38,39)
InChIKey IBSDUGROMOGHLR-UHFFFAOYSA-N
Boiling Point 785.9±70.0°C (Predicted)
Purity ≥95%
Density 1.23±0.1 g/cm3 (Predicted)
Related CAS 1643594-31-3 (polymer)
Appearance Light Yellow or White Solid
Storage Store at 2-8°C

Acid-PEG13-NHS ester, a versatile reagent widely employed in bioconjugation and biomedical research, holds immense potential in various applications. Here are four key applications, presented with high perplexity and burstiness:

Protein Labeling: Acid-PEG13-NHS ester serves as a cornerstone in protein labeling, allowing researchers to attach diverse tags, like fluorescent dyes or biotin, to proteins. This modification aids in visualizing and identifying proteins across a spectrum of assays, from Western blotting to immunoassays and microscopy. The PEG13 spacer, meticulously designed, ensures minimal steric hindrance while preserving the biological activity of the labeled proteins, enabling precise and reliable protein tracking.

Antibody Conjugation: At the forefront of targeted therapeutics and diagnostics, Acid-PEG13-NHS ester plays a pivotal role in conjugating antibodies to enzymes, drugs, or other molecules. This process is crucial for precise delivery and targeted binding in therapeutic and diagnostic applications. By forming stable amide bonds with primary amines in antibodies, Acid-PEG13-NHS ester facilitates the development of cutting-edge antibody-drug conjugates and immunoassays, elevating the specificity and efficacy of these biologically significant molecules.

Nanoparticle Functionalization: In the realm of nanomedicine, Acid-PEG13-NHS ester emerges as a key player in functionalizing nanoparticles for diverse biomedical applications, encompassing drug delivery, imaging, and biosensing. By attaching specific ligands or targeting molecules to the surface of nanoparticles, researchers can enhance the targeting efficiency and bioavailability of nanosystems dramatically. This precision in delivery ensures that therapeutics reach their intended destinations with heightened efficiency, minimizing off-target effects for improved patient outcomes.

Surface Modification: Crucial for enhancing the biocompatibility and functionality of biomaterials and medical devices, Acid-PEG13-NHS ester enables the modification of surfaces with bioactive molecules, like peptides or growth factors. This modification promotes crucial biological processes, such as cell adhesion, proliferation, and differentiation, pivotal in diverse applications ranging from tissue engineering to the development of advanced biosensors and implantable devices. The intricate linkages facilitated by Acid-PEG13-NHS ester play a vital role in shaping the future of biocompatible materials and medical technologies, driving innovations in healthcare and beyond.

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